Sonoporation: Gene transfer using ultrasound
نویسندگان
چکیده
منابع مشابه
Kidney-specific Sonoporation-mediated Gene Transfer
Sonoporation can deliver agents to target local organs by systemic administration, while decreasing the associated risk of adverse effects. Sonoporation has been used for a variety of materials and in a variety of organs. Herein, we demonstrated that local sonoporation to the kidney can offer highly efficient transfer of oligonucleotides, which were systemically administrated to the tubular epi...
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Microbubbles first developed as ultrasound contrast agents have been used to assist ultrasound for cellular drug and gene delivery. Their oscillation behavior during ultrasound exposure leads to transient membrane permeability of surrounding cells, facilitating targeted local delivery. The increased cell uptake of extracellular compounds by ultrasound in the presence of microbubbles is attribut...
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This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scl...
متن کاملSonoporation, a redefined ultrasound modality as therapeutic aid: A review
Traditionally a diagnostic modality, ultrasound is emerging as a promising tool for non-invasive therapy, drug delivery, and gene therapy. The ultrasound is a mechanical wave energy generated in a medium as oscillating pressure in space and time at frequencies above 20 kHz, beyond the audible range. The ultrasound exposure generates bioeffects resulting in tissue heating, shear stress, and cavi...
متن کاملOptimising ultrasound-mediated gene transfer (sonoporation) in vitro and prolonged expression of a transgene in vivo: potential applications for gene therapy of cancer.
Therapeutic approaches using gene-based medicines promise alternatives or adjuncts to conventional cancer treatment. Because of its non-invasive nature, ultrasound, as a membrane-permeabilising stimulus has the potential to be highly competitive with viral gene delivery and existing non-viral alternatives. In optimising ultrasound-mediated, microbubble-assisted (MB101) gene tranfection in vitro...
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ژورنال
عنوان ژورنال: World Journal of Methodology
سال: 2013
ISSN: 2222-0682
DOI: 10.5662/wjm.v3.i4.39